sem micrograph synthetic collagen-based materials col100  (BioMimetic Therapeutics)

Journal: Nature

Article Title: Mineralized collagen plywood contributes to bone autograft performance

doi: 10.1038/s41586-024-08208-z

Figure Lengend Snippet: a , b , SEM micrographs of decalcified human compact bone ( n = 1 sample) at low and high magnification, respectively. Scale bars, 50 μm ( a ), 5 μm ( b ). c , SEM micrograph of synthetic collagen-based materials characterized by a 3D dense and organized biomimetic structure (Col100; n = 3 samples). Scale bar, 5 μm. The plywood organization is depicted in the associated schematic representation below b . d , SEM micrograph of a non-organized collagen fibrillar network with large (micrometric) interfibrillar spaces (Col40; n = 3 samples). Scale bar, 2 μm.

Article Snippet: Scale bars, 50 μm ( a ), 5 μm ( b ). c , SEM micrograph of synthetic collagen-based materials characterized by a 3D dense and organized biomimetic structure (Col100; n = 3 samples).

Techniques:

Journal: Nature

Article Title: Mineralized collagen plywood contributes to bone autograft performance

doi: 10.1038/s41586-024-08208-z

Figure Lengend Snippet: a , SDS-PAGE of type I collagen extracted from rat tails tendons (1, n = 1 sample) and bovine dermis (commercialized by Symatese as clinical grade) (2, n = 1 sample), which confirms the higher purity of the commercial solution. The white double-headed arrow shows the band corresponding to the α chain of type I collagen (125.103 KDa). b , Differential scanning calorimetry of a collagen solution extracted from rat tail tendons (dashed line, 7 mg ml −1 ) and from bovine dermis (clinical grade) (solid line, 5 mg ml −1 ). The endothermic peak is seen at temperature around 40 °C and is typical of collagen denaturation into gelatine that occurs through the irreversible unfolding of the triple helix (ref. ). The purest collagen solution is the most stable, which may be because of a higher number of interactions between molecules. c , TEM observations of an ultrathin section of clinical-grade collagen fibrils showing the typical cross-striated pattern ( n = 3 samples). d , d′ , Polarized light microscopy of clinical-grade collagen solution (concentration approximately 80 mg ml −1 ) observed between crossed polars (at 0° ( d ) and at 45° ( d′ )) and showing the typical birefringence of cholesteric order (alternating bright and dark bands). e , Picture of a ColCG-CHA matrix. f , SEM micrograph of a ColCG-CHA matrix ( n = 1 sample) showing a periodic stratification typical of the twisted plywood organization. g , TEM micrograph of a ColCG-CHA matrix ( n = 1 sample) showing the co-alignment between apatite platelets and the main axis of collagen fibrils.

Article Snippet: Scale bars, 50 μm ( a ), 5 μm ( b ). c , SEM micrograph of synthetic collagen-based materials characterized by a 3D dense and organized biomimetic structure (Col100; n = 3 samples).

Techniques: SDS Page, Differential Scanning Calorimetry, Light Microscopy, Concentration Assay